A new method to detect infections and to test serious diseases and drugs is developed by Manchester University scientists which uses Bio chips.
Published online in the Journal of the American Chemical Society (JACS), the technique uses Protein chips that are objects like slides. They have proteins attached to them and permit crucial data about the behavior of proteins to be gathered. They permit to conduct tests to understand the behavior of bio proteins and their interaction with cells, drugs, DNA and other proteins simultaneously.
The Manchester team of Jenny Thirlway and Jason Micklefield and Lu Shin Wong, said “the technical challenges of attaching proteins in a reliable way have previously held back the widespread application and development of protein chips.”
As proteins can be placed precisely on a chip, it would be possible to scan large numbers of them simultaneously and also isolate the data bearing on individual proteins.
The benefit of using these chips is that they uses a very minimum amount of material- the protein which makes an easy task to gather information about rare proteins which are available in a very small amount.
According to the researchers they have found a better way to attach active protein to a chip which is reliable, quick and efficient. Unlike the traditional method, the attachment in the new method occurs in a single step in just a few hours.
It requires no prior chemical modification of the protein of interest or additional chemical steps.
Researcher Jason Micklefield said, “DNA chips have revolutionized biological and medical science. For many years scientists have tried to develop similar protein chips but technical difficulties associated with attaching large numbers of proteins to surfaces have prevented their widespread application.”
“The method we have developed could have profound applications in the diagnosis of disease, screening of new drugs and in the detection of bacteria, pollutants, toxins and other molecules,’ Micklefield added.”